Composite

Part:BBa_K4685000:Design

Designed by: Asia Andreoli   Group: iGEM23_UNILausanne   (2023-10-10)


SpyCatcher fused to gene Als3 ( Rubber-binding protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1462
    Illegal PstI site found at 361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1462
    Illegal PstI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1427
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1462
    Illegal PstI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1462
    Illegal PstI site found at 361
    Illegal NgoMIV site found at 1161
    Illegal AgeI site found at 1294
    Illegal AgeI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design consideration


Source

In literature, we found a protein domain (Als3) that has been shown to bind to rubber (Zhao et al., 2006)

References

Zhao, X., Daniels, K.J., Oh, S.-H., Green, C.B., Yeater, K.M., Soll, D.R., Hoyer, L.L., 2006. Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces. Microbiology 152, 2287–2299. https://doi.org/10.1099/mic.0.28959-0