![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K4683028:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4683028
Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
User Reviews
UNIQdcefca5e7bd63b0b-partinfo-00000001-QINU UNIQdcefca5e7bd63b0b-partinfo-00000002-QINU