DNA

Part:BBa_K4683001

Designed by: Natalie Tan   Group: iGEM23_Lambert-GA   (2023-10-10)


Nb.BbvCI nicking enzyme cut site

This part is the site for endonclease Nb.BbvCI to generate DNA molecules that are nicked.



References Li, X.-Y., Du, Y.-C., Zhang, Y.-P., & Kong, D.-M. (2017). Dual functional phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-06594-1
https://www.neb.com/en-us/products/r0631-nbbbvci#Product%20Information


Sequence and Features CCTCAGC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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