Coding

Part:BBa_K4680009:Design

Designed by: Chase Tarillo   Group: iGEM23_FSU   (2023-07-07)


Human Flavin containing Dimethylaniline Monoxygenase 3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1150
    Illegal PstI site found at 1513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1150
    Illegal PstI site found at 1513
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1150
    Illegal PstI site found at 1513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1150
    Illegal PstI site found at 1513
    Illegal NgoMIV site found at 864
    Illegal AgeI site found at 364
    Illegal AgeI site found at 843
    Illegal AgeI site found at 985
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Flavin containing dimethylaniline monooxygenase 3 (FMO3) sequence is from the Homo sapien species and codes for the FMO3 enzyme. This enzyme is responsible for the oxidation of TMA (trimethylamine) into TMAO (trimethylamine N-oxide) in the liver. This sequence was taken from the AUC Turkey Human FMO3 sequence (BBa_K727004) but modified by taking out the BioBrick prefix and suffix.


Source

GenBank: https://www.ncbi.nlm.nih.gov/gene/2328

References

GenBank: https://www.ncbi.nlm.nih.gov/gene/2328