Generator

Part:BBa_K4680008:Design

Designed by: Chase Tarrillo   Group: iGEM23_FSU   (2023-06-06)


FMO3 Homo S. Generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1220
    Illegal PstI site found at 1583
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1220
    Illegal PstI site found at 1583
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1220
    Illegal PstI site found at 1583
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1220
    Illegal PstI site found at 1583
    Illegal NgoMIV site found at 934
    Illegal AgeI site found at 434
    Illegal AgeI site found at 913
    Illegal AgeI site found at 1055
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biological device consists of an Anderson promoter (J23108), synthetic RBS (K1403008), human flavin containing dimethylaniline monooxygenase (K4680009), and a terminator (B1006). These basic pieces we used to have a functional device.

Source

GenBank: https://www.ncbi.nlm.nih.gov/gene/2328

References

GenBank: https://www.ncbi.nlm.nih.gov/gene/2328