Generator

Part:BBa_K4680004:Design

Designed by: Chase Tarrillo   Group: iGEM23_FSU   (2023-06-06)


R. pomeroyi TMM Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biological device consists of an Anderson promoter (J23108), synthetic RBS (K1403008), the Paris Bettencourt Ruegeria pomeroyi trimethylamine monooxygenase (K1403007), and a terminator (B1006). These basic pieces we used to have a functional device.

Source

Wiki: https://2014.igem.org/Team:Paris_Bettencourt/Parts

References

Wiki: https://2014.igem.org/Team:Paris_Bettencourt/Parts