R. pomeroyi TMM Generator
Usage and Biology
The FSU iGEM team created this biological device to oxide trimethylamine (TMA) in the small intestine of a human. The purpose behind this device is to eliminate TMA from the body for those struggling with trimethylaminuria (TMAU). TMAU is a rare condition (or disease) in which a mutation appears on the FMO3 enzyme coding sequence and the body can no longer oxidize trimethylamine into trimethylamine N-oxide in the liver. This causes the person to expel a fishy odor throughout their entire body. Our device consists of an Anderson promoter, synthetic RBS, a TMM enzyme coding sequence taken from the Paris Bettencourt 2014 team, and a terminator. The trimethylamine monooxygenase coding sequence comes from a species called Ruegeria Pomeroyi. This species comes from the Marine Roseobacter clade that uses TMAO as a nutrient source. The Vmax and Km values of the TMM enzyme are 15.8 +/- 3.1 μg*min-1*mg-1 and 20.8 +/- 2.9 μM. These values are higher than the human FMO3 enzyme values, therefore we assume it would work better than the human FMO3 enzyme.
This biological device has been inserted in a pIDTSmart-Kan plasmid backbone from IDT resulting in pFSUIGEM201. Figure 1 depicts the map of pFSUIGEM201.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BamHI site found at 432
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 256
Illegal AgeI site found at 479
- 1000COMPATIBLE WITH RFC