Composite

Part:BBa_K4674011:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


The RepA and SSBP expressing cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1230
    Illegal XhoI site found at 1225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nope


Source

This part comprises T7 promoter, Lac operator, RBS, 6xHis-tag, Thrombin cleavage site, SSBP gene, RepA gene, and T7 terminator for protein expression

References