Coding
SSBP

Part:BBa_K4674003:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


Single-strand binding protein (SSBP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part is codon optimized for E.Coli expression.


Source

The SSBP is originated from E. coli. The protein sequence could be obsatined from GenBank as QGY26441.1

References

Alexander G, et. al. Binding Specificity of E. coli SSB protein for the χ subunit of DNA pol III Holoenzyme and PriA helicase. Biochemistry. 2010 May 4; 49(17): 3555–3566.

Marceau AH, et al. Structure of the SSB--DNA polymerase III interface and its role in DNA replication The Embo Journal, 30(20), 4236-4247 (2011)