Coding

Part:BBa_K4674001:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-09-30)


mGL-4A-C7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The design of mGL-4A-C7

To efficiently label the CTCs captured by DNA tetrahedron, we first perform modeling and determine to link mGL protein and C7 dedocapeptide with 4 alaines (mGL-4A-C7). To expression the mGL-4A-C7 fusion protein, we design and cloned the composite par BBa_K4674010 into the pET15b. The fusion protein was induced by IPTG and purified by FPLC. Finally, to confirm whether the mGL-4A-C7 is functional, we perform the labeling experiments by SKOV3 cell line, which is the mimic of the captured CTC. (Due to the highly expression of FRα in SKOV3 cells)

fig-9-design-okik-2.png


Source

The mGL is derived from Aequorea victoria, while the C7 is a synthetic peptide.

References