DNA

Part:BBa_K4673031:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)


b-lactamase coding region is an ampicillin resistance gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 542
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 542
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716


Design Notes

Ampicillin promoter is located between T7 terminator and ampicillin resistance in the pET-22b LK vector, and located between the b-lactamase coding region and f1-origin in the pGEM-T EASY vector.


Source

The b-lactamase coding region is also on the pGEM-T EASY vector purchased from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

References

Bush, K., & Bradford, P. A. (2016, August 1). Β-lactams and β-lactamase inhibitors: An overview. Cold Spring Harbor perspectives in medicine. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968164/