Part:BBa_K4673022:Design

Lac promoter binds RNA polymerase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The Lac promoter is located between the lac operon and the lac operator in the pGEM-T EASY vector.

Source

The lac promoter is one of the components of the pGEM-T EASY vector, which our team bought from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

In addition, the lac promoter is also on the pET-22b-LK. GSL Biotech LLC. (2023). pET-22b(+) Sequence and Map. SnapGene. https://www.snapgene.com/plasmids/pet_and_duet_vectors_(novagen)/pET-22b(%2B)

References

Sudheer Menon, Shanmughavel Piramanayakam, & Gopal Agarwal. (2021). Computational identification of promoter regions in prokaryotes and eukaryotes. EPRA International Journal of Agriculture and Rural Economic Research, 21–28. https://doi.org/10.36713/epra7667