Regulatory

Part:BBa_K4673018:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)


Lac operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Lac operon is located between the f1 _origin and lacZ_a on the pET-22b vector.


Source

In terms of our project, the lac operator is composed by team Taipei_KCISLK_V1, which does not come from genomics in this case.


References

Hediger, M. A., Nierlich, D. P., & Zabin, I. (1985, October). DNA sequence of the lactose operon: The laca gene and the transcriptional termination region. Proceedings of the National Academy of Sciences of the United States of America. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390726/