Composite
R12-Ftn

Part:BBa_K4669006:Design

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-10-04)


R12-Ferritin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cell penetrating peptide should be located at the N-terminus, because the C-terminus will be inside after container ferritin assembled and the peptide would not be able to act since it can not reach the cell wall. Also, the N-terminus of the TAT peptide must not be blocked by anything else. Even though the exact mode of action is not know yet, it is know that a free N-terminus is essential.


Source

R12 is synthetic, the sequence for ferritin is obtained by the human heavy chain protein.

References