DNA

Part:BBa_K4663026

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


dapB

dapB encodes for dihydrodipicolinate reductase, an enzyme playing a key role in the biosynthesis of DAP which serves as a direct precursor of lysine. Both compounds are necessary for the survival of bacterial cells, therefore a knockout of dapB results in a strain auxotrophic for both compounds.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 205
    Illegal NgoMIV site found at 75
    Illegal AgeI site found at 301
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None