DNA

Part:BBa_K4663024

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


rhaSR-PrhaBAD

rhaSR-PrhaBAD is an inducible system originating from E. coli allowing L-rhamnnose- and L-mannose-inducible gene expression. In order to achieve this effect in other bacteria than E. coli, this entire system has to be cloned upstream of the gene of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1995
    Illegal NheI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1995
    Illegal BglII site found at 1315
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1995
    Illegal AgeI site found at 1583
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None