Part:BBa_K4661000:Design

HFBI_Ecoli_optimized

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 154
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimized sequence of HFBI from Trichoderma reesei for E coli expression, adapted GC content at 5' and 3' ends to facilitate PCR amplification. Designed to work with both Gibson and Type IIs assembly methods. Part of library to hydrophobin sequences further amplified with primers designed to make part compatible with pET29 plasmid expression system.

Source

Synthesized as gBlock from IDT.

GenBank 2FZ6_A

References

Lupeng Cui, Cheng Cheng, Yumeng Qiu, Tianyue Jiang, Bingfang He, Excretory overexpression of hydrophobins as multifunctional biosurfactants in E. coli, International Journal of Biological Macromolecules, Volume 165, Part A, 2020, Pages 1296-1302, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2020.09.206. (https://www.sciencedirect.com/science/article/pii/S0141813020345621)