Composite

Part:BBa_K4656007:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-09)


Plam-TPH-TDC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2641
    Illegal BamHI site found at 2125
    Illegal BamHI site found at 2227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2168
    Illegal NgoMIV site found at 2723
    Illegal AgeI site found at 734
    Illegal AgeI site found at 1871
    Illegal AgeI site found at 2795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 301


Design Notes

The promoter Plam can be inhibited by binding to CI repressor proteins.


Source

Tph1 gene sequence is from Homo sapiens.

CI repressor protein is known to be derived from E. coli phage (+LVA).

References