Part:BBa_K4654011:Design
Li+-II_Riboswitch
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 40
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When incorporating the riboswitch in constructs like our composite part BBa_K4654018, we made sure to include the spacer sequences up- and downstream of the riboswitch to preserve the natural environment of the riboswitch, because they influence the folding of the riboswitch.
Figure 1: This figure shows the riboswitch and its flanking sequences.
Source
The riboswitch can be found in certain bacteria, the sequence we are using was created by White et. al. by creating a consenus sequence from different sequences of the riboswtich family1.
References
1 White, N., Sadeeshkumar, H., Sun, A. et al. Lithium-sensing riboswitch classes regulate expression of bacterial cation transporter genes. Sci Rep 12, 19145 (2022). https://doi.org/10.1038/s41598-022-20695-6
.