Part:BBa_K4654010:Design
Spacer Sequence 2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
An important insight into the design of the construct came from the expertise of Prof. Dr. Mario Moerl, who emphasized the importance of maintaining the natural environment of the riboswitch (BBa_K4654011). This is crucial because it influences the folding and functionality of the riboswitch. We retained the spacer sequence containing the ribosome binding site (RBS) and the 5' region of the nhaA gene between the riboswitch and the reporter sequence. For this, we used the same sequence configuration that White et al. (2022)1 used in their experiments.
Source
The sequence we are using was created by White et. al. by creating a consenus sequence from different sequences of the riboswtich family1.
References
1 White, N., Sadeeshkumar, H., Sun, A. et al. Lithium-sensing riboswitch classes regulate expression of bacterial cation transporter genes. Sci Rep 12, 19145 (2022). https://doi.org/10.1038/s41598-022-20695-6