Composite

Part:BBa_K4653113:Design

Designed by: Tangkun Zhu   Group: iGEM23_SZU-China   (2023-10-10)


T7 promoter - BvEP - 6x His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 187
    Illegal BsaI.rc site found at 281


Design Notes

The recombinant plasmid was transferred into the proteinase-deficient Escherichia coli BL21 (DE3), and the fermentation of BvEP was induced by IPTG.


Source

The obtained target gene sequence was assembled into the polyclonal site of the pET28a (+) plasmid, and the 6*His protein purification tag sequence was linked to facilitate subsequent protein extraction and purification experiments.

References