Coding
Part:BBa_K4652000:Design
Designed by: YUAN-AN CHEN Group: iGEM23_Mingdao (2023-08-14)
SpyTag-GFP-SpyCatcher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 701
Design Notes
No
Source
Synthesized by Integrated DNA Technologies, Inc. The DNA sequence for GFP is from Part:BBa_E0040 except deletion of start codon (ATG) and stop codon (TAA). SpyTag and SpyCatcher are from CnaB domains of fibronectin-binding (FbaB) protein in Streptococcus pyogenes.