Part:BBa_K4650005

BBa_K4650005 contained BBa_K4650000+BBa_K4650002+BBa_K4650001 (pGal1,10-MaSp1-CBD) and was transformed into BY4741 to make the BY4741 containing BBa_K4650005 as one of the experimental strains in our project. MaSp1 encodes for spider silk, which is a highly repetitive amino acid sequence and has the properties of being strong, flexible, and elastic. This amino acid sequence is one of the shortest compared to other amino acid sequences for spider silk proteins listed in the paper by Tian et al, 2003. The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes to make our team’s scoby membranes more flexible and softer.