Primer

Part:BBa_K4649011:Design

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-11)


SK Primer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 12
    Illegal SpeI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 6
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 12
    Illegal SpeI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 12
    Illegal SpeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to have a sequence that is the reverse complement of a region of template or target DNA to which we wish the primer to anneal.


Source

pBBR1MCS-2

References