Composite

Part:BBa_K4647031

Designed by: Bushra Islam   Group: iGEM23_Stony-Brook   (2023-10-12)


nif XAV with IVA overhangs

Nif gene chunk with nifX, hesA, and nifV assembled with IVA overhangs, which can be used in combination with nif BHD and nif KEN to assemble minimal nitrogen fixation gene cluster for the expression of nitrogenase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


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Parameters
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