Coding
Part:BBa_K4644004:Design
Designed by: Han Cao Group: iGEM23_BUCT (2023-09-23)
pgsBCAE
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 737
Illegal XbaI site found at 1032
Illegal PstI site found at 382
Illegal PstI site found at 1013
Illegal PstI site found at 2028 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 737
Illegal PstI site found at 382
Illegal PstI site found at 1013
Illegal PstI site found at 2028 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 737
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 737
Illegal XbaI site found at 1032
Illegal PstI site found at 382
Illegal PstI site found at 1013
Illegal PstI site found at 2028 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 737
Illegal XbaI site found at 1032
Illegal PstI site found at 382
Illegal PstI site found at 1013
Illegal PstI site found at 2028
Illegal NgoMIV site found at 1277
Illegal AgeI site found at 2968 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed PETlac-pgsBCAE plasmid
Source
Providencia rettgeri strain JNB815.Sequence found from Genbank https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP029736.1?report=fasta&from=1655614&to=1655937&strand=true
References
Bai, N.; He, Y.; Zhang, H.; Zheng, X.; Zeng, R.; Li, Y.; Li, S.; Lv, W. γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of Bacillus subtilis A-5 and the Complete Genome Analysis. Int. J. Environ. Res. Public Health 2022, 19, 7630.
Xiaoyu Wei, Zhen Chen, Ailing Liu, Lijie Yang, Yiyuan Xu, Mingfeng Cao, Ning He,Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances,Biotechnology Advances,Volume 67,2023,108199,ISSN 0734-9750,