Part:BBa_K4643010

Cytochrome P450 52-M1

Catalyzes the first step of sophorolipid biosynthesis. Catalyzes the terminal (at the omega-position) or subterminal (at the omega-1-position) hydroxylation of a fatty acid. This converts the fatty acid to a substrate for the subsequent glycosyltransferase reactions (PubMed:23516968, PubMed:24242247). Oleic acid is the preferred substrate, but it acts on various other C-16, C-18 and C-20 saturated and unsaturated fatty acids, namely palmitic, palmitoleic, stearic, linoleic, cis-9,10-epoxystearic, trans-9,10-epoxystearic and arachidonic acid (PubMed:24242247). Optimum pH is 7 Optimum temperature is 25-30 degrees Celsius

Uniprot link : https://www.uniprot.org/uniprotkb/B8QHP1/entry

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 619
  Illegal EcoRI site found at 796
  Illegal EcoRI site found at 1369
  Illegal PstI site found at 908
  Illegal PstI site found at 1504
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 619
  Illegal EcoRI site found at 796
  Illegal EcoRI site found at 1369
  Illegal PstI site found at 908
  Illegal PstI site found at 1504
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 619
  Illegal EcoRI site found at 796
  Illegal EcoRI site found at 1369
  Illegal BglII site found at 308
  Illegal BamHI site found at 1278
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 619
  Illegal EcoRI site found at 796
  Illegal EcoRI site found at 1369
  Illegal PstI site found at 908
  Illegal PstI site found at 1504
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 619
  Illegal EcoRI site found at 796
  Illegal EcoRI site found at 1369
  Illegal PstI site found at 908
  Illegal PstI site found at 1504
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 66