Protein_Domain

Part:BBa_K4643004

Designed by: Baptiste Rivoirard   Group: iGEM23_Sorbonne-U-Paris   (2023-09-24)


pBBR1 Replication protein

replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica

The replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica is known as RepB. RepB is a replication initiator protein that plays a crucial role in initiating and controlling plasmid replication. This plasmid is widely used in molecular biology and biotechnology research because of its ability to replicate in a broad range of bacterial hosts. RepB recognizes and binds to specific DNA sequences in the plasmid's origin of replication, which then triggers the initiation of DNA replication, allowing the plasmid to replicate autonomously within the host bacterial cell. This feature makes pBBR1 a valuable tool for genetic engineering and the expression of genes in a variety of bacterial species.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None