Part:BBa_K4636036:Design

PCRforAELA_fw

Design

We used Primer 3[1] to search the suitable site and check it by IDT OligoAnalyzer™ Tool.[2] General considerations for PCR Primers.[3]

(1) : Primer size between 25~30 bps.

(2) : Value of GC content should be 40%~60%.

(3) : 3’ end with highly GC content.

(4) : Tm value between 50~60°C.

Reference