Composite
pAND

Part:BBa_K4634013:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-06)


Single Plasmid AND GATE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2502
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1245
    Illegal AgeI site found at 2261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1248
    Illegal SapI site found at 961


Design Notes

NA


Source

NA

References

[1] Anderson JC, Voigt CA, Arkin AP. Environmental signal integration by a modular AND gate. Mol Syst Biol. 2007;3:133. doi: 10.1038/msb4100173. Epub 2007 Aug 14. PMID: 17700541; PMCID: PMC1964800.