Composite

Part:BBa_K4634011

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-06)


OmpA-mCherry

The OmpA signal peptide(BBa_K4380001) is a short amino acid sequence found at the N-terminus of the OmpA protein in Gram-negative bacteria. The signal peptide acts as a recognition signal for the protein translocation machinery present in the bacterial cell. In the composite part OmpA-mCherry, 21aa long OmpA signal peptide is linked at the N terminal of mCherry(BBa_K2244008), which is used to test the function of OmpA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.

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