Composite

Part:BBa_K4634009:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-04)


Temperature Control Induction Reporter System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1324
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 838
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1324
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1324
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

Zhang ZQ, Yao LH, Hou YD. Construction and application of a high level expression vector containing PRPL promoter. Chinese Journal of Virology, 1990, 6(2): 111−116.

References

[1] Ju, L.W., et al., GeneDn: for high-level expression design of heterologous genes in a prokaryotic system. Bioinformatics (Oxford, England), 1998. 14(10): p. 884-885.
[2] Abedi, M.H., et al., Ultrasound-controllable engineered bacteria for cancer immunotherapy. Nature Communications, 2022. 13(1): p. 1585.
[3] Chen, Y., et al., Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy. Nature Communications, 2022. 13(1): p. 4468