Regulatory

Part:BBa_K4634000:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-09-25)


pPepT: hypoxia-inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Get from paper: Chien, T., Harimoto, T., Kepecs, B. et al. Enhancing the tropism of bacteria via genetically programmed biosensors. Nat Biomed Eng 6, 94–104 (2022). https://doi.org/10.1038/s41551-021-00772-3

References

[1]Yu, Bin, et al. "Explicit hypoxia targeting with tumor suppression by creating an “obligate” anaerobic Salmonella Typhimurium strain." Scientific reports 2.1 (2012): 436. [2]Crack, Jason C., et al. "Signal perception by FNR: the role of the iron sulfur cluster1." (2008): 1144-1148. [3] CRACK J C, GASKELL A A, GREEN J, et al. Influence of the Environment on the [4Fe−4S]2+ to [2Fe−2S]2+ Cluster Switch in the Transcriptional Regulator FNR [J]. Journal of the American Chemical Society, 2008, 130(5): 1749-58.