![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4633008:Design
Designed by: Mor Moshe Group: iGEM23_Technion-Israel (2023-09-26)
Colicin D - Bacteriocin from E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1273
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1524
Illegal AgeI site found at 506
Illegal AgeI site found at 1470 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1725
Design Notes
This part was meant to be expressed in B. Subtilis 168, the Gram-Positive bacteria.
Source
The sequence of Colicin D was derived from the genome of E.coli [1].
References
- [1] U. Roos, R. E. Harkness, and V. Braun, “Assembly of colicin genes from a few DNA fragments. Nucleotide sequence of colicin D,” Mol Microbiol, vol. 3, no. 7, pp. 891–902, 1989, doi: 10.1111/J.1365-2958.1989.TB00238.X