Coding

Part:BBa_K4633008:Design

Designed by: Mor Moshe   Group: iGEM23_Technion-Israel   (2023-09-26)


Colicin D - Bacteriocin from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1273
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1524
    Illegal AgeI site found at 506
    Illegal AgeI site found at 1470
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1725


Design Notes

This part was meant to be expressed in B. Subtilis 168, the Gram-Positive bacteria.


Source

The sequence of Colicin D was derived from the genome of E.coli [1].


References

  • [1] U. Roos, R. E. Harkness, and V. Braun, “Assembly of colicin genes from a few DNA fragments. Nucleotide sequence of colicin D,” Mol Microbiol, vol. 3, no. 7, pp. 891–902, 1989, doi: 10.1111/J.1365-2958.1989.TB00238.X