Part:BBa_K4632003:Design

Cowpea trypsin inhibitor(CPTI)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Differing from the original study (Wei Gao et al., 2013), we did not fuse the CPTI gene with the GST purification tag for expression. Our goal is to directly utilize CPTI expression for the eradication of Solenopsis invicta, without the subsequent removal of the GST tag. Moreover, if the CPTI lacking the GST tag can be successfully expressed, its protein molecular weight would be only 10.4 kDa.

Following Tricine-PAGE and Western Blot experiments, the results indicate that the CPTI gene without the GST tag does not express as expected, as described in the literature. In the next step, we have constructed the CPTI gene with the GST tag, and protein induction expression is currently underway.

Source

Wei Gao, Feng Li, Qingyu Lu. CPTI (Cowpea Trypsin Inhibitor) gene, applications thereof and method for preparing great amount of CPTI: CN102127552B[P]. 2013-02-13.

References

[1]Xuhong-lin, Zhaihong-li, Wangfeng et al. Cowpea Trypsin Inhibitor Gene(cpti) and its Application in Insect Resistance Transgenic plants[J]. 2008.

[2] Wei Gao, Feng Li, Qingyu Lu. CPTI (Cowpea Trypsin Inhibitor) gene, applications thereof and method for preparing great amount of CPTI: CN102127552B[P]. 2013-02-13.