Composite

Part:BBa_K4630403:Design

Designed by: Zixuan Ding   Group: iGEM23_WHU-China   (2023-10-08)


N20 screening-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1394
    Illegal SpeI site found at 970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1493
    Illegal NheI site found at 2004
    Illegal NheI site found at 2014
    Illegal NheI site found at 2073
    Illegal SpeI site found at 970
    Illegal NotI site found at 541
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 173
    Illegal XhoI site found at 534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1394
    Illegal SpeI site found at 970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1394
    Illegal SpeI site found at 970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 993
    Illegal BsaI.rc site found at 1755


Design Notes

Fragment loss sometimes happened after successful construction.


Source

We built this composite part based on EGFP-N20s (BBa_K4630401) and N20s screening-LacZ (BBa_K4630402).

References