Measurement

Part:BBa_K4630400:Design

Designed by: Zixuan Ding   Group: iGEM23_WHU-China   (2023-10-08)


LacZ inserted with 50*( N20+PAM)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1401
    Illegal XbaI site found at 482
    Illegal XbaI site found at 1430
    Illegal SpeI site found at 58
    Illegal PstI site found at 1458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1401
    Illegal NheI site found at 581
    Illegal NheI site found at 1092
    Illegal NheI site found at 1102
    Illegal NheI site found at 1161
    Illegal SpeI site found at 58
    Illegal PstI site found at 1458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1401
    Illegal BamHI site found at 1440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1401
    Illegal XbaI site found at 482
    Illegal XbaI site found at 1430
    Illegal SpeI site found at 58
    Illegal PstI site found at 1458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1401
    Illegal XbaI site found at 482
    Illegal XbaI site found at 1430
    Illegal SpeI site found at 58
    Illegal PstI site found at 1458
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 81
    Illegal BsaI.rc site found at 843


Design Notes

The synthesis of N20s is not easy. You should choose a reliable biotech company or use other methods like Golden Gate Assembly to generate the sequence.


Source

it's constructed by inserting a synthesized sequence, N20s, into pUC57 vector.

References