Part:BBa_K4630301

EL222,blue light activated inducible system with RFP reporter

EL222, a blue-light inducible promoter, is a typical single-component photosensitive system. pEL222 is a gene segment that can bind to EL222 protein. In the darkness, the EL222 protein remains monomer without binding to pEL222 and inactivates the downstream gene. While under the blue light, the two monomers are polymerized into a dimer and the dimer binds to pEL222, activating downstream genes. Results of the previous teams have shown that two EL222 monomers can still bind together even in the darkness, causing downstream leakage.

Figure. 1 Image results of induced mRFP1 expression in pET-28a- [EL222 new-mRFP1] transformant, shot at 1/15S, ISO 2000.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 130
  Illegal AgeI site found at 355
  Illegal AgeI site found at 1503
  Illegal AgeI site found at 1615
  
• 1000
  COMPATIBLE WITH RFC[1000]