Part:BBa_K4630117
Xbal-stgframe-Spel-BamHI-selfRecombination
Xbal-stgframe-Spel-BamHI-selfRecombination is the cassette for illumination form of self-targeting guide RNA constituted by N20, which can be designed and developed independently. This part can be transcripted as a piece of guide RNA to target its own fragment combined with N20s and achieve a knock-out. Then Lambda system can implement the repair of the knockout fragment later. If the repair has been done correctly, the fragment can be connected and the green fluorescent protein gene can be expressed. Then we will observe green fluorescence. In other words, we can identify whether the fragment has been knocked out and repaired.
It is worth mentioning that N20 fragments through computer design, we can design a variety of N20 fragments, to change the knockout rate, to change the knocked-out fragments, so as to achieve the control of gene knockout.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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