DNA

Part:BBa_K4630101

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


stgRNA-barcode-cassette 2

This part is the barcode-cassette form of the self-targeting guide RNA 2 (stgRNA 2). It is the building block of the cascade recording system, making it extendable. It contains unique barcode, self-targeting guide RNA (stgRNA 2), double terminator and the barcode. It is used in the verification of the self-targeting and self-recombination system. The unique barcode is used to distinguish the cassette and provide unique homologous arm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None