DNA

Part:BBa_K4630001:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


self-targeting guide RNA 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold downstream the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.

Source

Complete synthesis.


References

1. Perli SD, Cui CH, Lu TK. Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016;353(6304):aag0511. doi:10.1126/science.aag0511