![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4629001:Design
Designed by: Anika Kapur, Thanakorn (Gunn) Vajirakachorn, Ananya Dharna, Vin Pungprasert, Dhirath (Kaka) Thanglerdsumpan Group: iGEM23_Thailand-RIS (2023-10-10)
Durio zibethinus glutamate-cysteine ligase, mitochondrial
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1125
Illegal SapI.rc site found at 1420
Design Notes
The mitochondrial transit peptide was obtained from Arabidopsis thaliana and was inserted, thus replacing the chloroplast transit peptide.
Source
The Durio zibethinus GCL gene is obtained from GenBank (gene ID: LOC111291428) coding sequence (XM_022883172.1; https://www.ncbi.nlm.nih.gov/nuccore/XM_022883172.1?from=132&to=1712). The gene was modified at the 5'-terminus with Arabidopsis thaliana mitochondrial succinate dehydrogenase iron-sulphur subunit (AJ278910.1; https://www.ncbi.nlm.nih.gov/nuccore/AJ278910.1?report=genbank&from=13&to=852) to change the subcellular localization from chloroplast to mitochondria.