Part:BBa_K4628047

50Spro-KillerRed

KillerRed (BBa_K1184000) is a red fluorescent protein that generates reactive oxygen species (ROS) under yellow-green light (540-585 nm). This component serves a crucial role in preventing engineered bacteria from inadvertently escaping into the environment.

Usage and Biology

In our experiments, we coupled the KillerRed protein with the 50Spro promoter and terminator BBa_B0015. These elements were cloned into plasmid pGreenⅡ and subsequently transformed into Agrobacterium tumefaciens GV3101 for further examination.

Result：

Our experiments with the bacterial solution under light exposure revealed that the solution did not exhibit significant reduction. We hypothesize that the accumulation of toxins within the bacteria may have a buffering effect in a liquid environment.

In contrast, our plate light experiment demonstrated a noticeable reduction of engineered bacteria under natural light irradiation, regardless of whether the conditions were sunny or cloudy.

FIG. Results of light experiments of Agrobacterium with KillerRed V.S. empty plasmid

Reference

[1] A D G ,B D S ,A K L , et al. Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity.[J]. International journal of molecular sciences,2020,21(22).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 577
  Illegal BsaI.rc site found at 868