Generator

Part:BBa_K4628029:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Pikm-2 expression circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2947
    Illegal PstI site found at 1391
    Illegal PstI site found at 2799
    Illegal PstI site found at 2921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2947
    Illegal PstI site found at 1391
    Illegal PstI site found at 2799
    Illegal PstI site found at 2921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2947
    Illegal BglII site found at 3743
    Illegal BamHI site found at 2261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2947
    Illegal PstI site found at 1391
    Illegal PstI site found at 2799
    Illegal PstI site found at 2921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2947
    Illegal PstI site found at 1391
    Illegal PstI site found at 2799
    Illegal PstI site found at 2921
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Combined with CaMV 35S promoter, Pikm-2, CaMV poly(A) signal

References