Generator

Part:BBa_K4628028

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)

Pikm-1(Enhancer) expression circuit

Combined with CaMV 35S promoter, Pikm-1(Enhancer), CaMV poly(A) signal

Usage and Biology

Initiate the expression of the fusion protein Pikm-1-Enhancer with a strong promoter CaMV. This fusion protein combines the ability of Pikm-1 to activate the endogenous immune pathway in plants with the ability of Enhancer to specifically target GFP, allowing us to mediate a strong immune response in plants through the stimulation of common reporter protein GFP, and to measure the immune intensity of plants through the clearance rate of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3973
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3079
    Illegal BamHI site found at 2704
    Illegal XhoI site found at 3537
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 1090
    Illegal NgoMIV site found at 2458
    Illegal NgoMIV site found at 3555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2688
    Illegal SapI site found at 1626
    Illegal SapI.rc site found at 885
    Illegal SapI.rc site found at 3596


[edit]
Categories
Parameters
biologyArtificial synthesis
chassisNicotiana benthamiana