Part:BBa_K4628027

Pikm-1 expression circuit

Combined with CaMV 35S promoter, Pikm-1, CaMV poly(A) signal

Usage and Biology

The expression of plant NLR molecule Pikm-1 is initiated by the CaMV strong promoter, which has natural binding ability to rice blast effectors and can initiate downstream immunity in plants.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1721
  Illegal SpeI site found at 2295
  Illegal SpeI site found at 2301
  Illegal PstI site found at 2514
  Illegal PstI site found at 2730
  Illegal PstI site found at 2745
  Illegal PstI site found at 2814
  Illegal PstI site found at 3258
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3844
  Illegal SpeI site found at 2295
  Illegal SpeI site found at 2301
  Illegal PstI site found at 2514
  Illegal PstI site found at 2730
  Illegal PstI site found at 2745
  Illegal PstI site found at 2814
  Illegal PstI site found at 3258
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2950
  Illegal BamHI site found at 2575
  Illegal XhoI site found at 3408
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1721
  Illegal SpeI site found at 2295
  Illegal SpeI site found at 2301
  Illegal PstI site found at 2514
  Illegal PstI site found at 2730
  Illegal PstI site found at 2745
  Illegal PstI site found at 2814
  Illegal PstI site found at 3258
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1721
  Illegal SpeI site found at 2295
  Illegal SpeI site found at 2301
  Illegal PstI site found at 2514
  Illegal PstI site found at 2730
  Illegal PstI site found at 2745
  Illegal PstI site found at 2814
  Illegal PstI site found at 3258
  Illegal NgoMIV site found at 2329
  Illegal NgoMIV site found at 3426
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1142
  Illegal BsaI site found at 2559
  Illegal BsaI.rc site found at 1096
  Illegal SapI site found at 1497
  Illegal SapI.rc site found at 885
  Illegal SapI.rc site found at 3467