Regulatory

Part:BBa_K4628024:Design

Designed by: Junjie Li, Zhijian Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Ptac/cuo


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We hope to synthesize a promoter strong enough and manipulable to control suicide gene expression in Agrobacterium, but further experimental studies are needed to characterize this promoter.


Source

We obtained this fragment by Gene Synthesis Services and overlap extension PCR and ligated it to the pGreenII plasmid by Gibson assembly.

References