Part:BBa_K4628024

Ptac/cuo

Usage and Biology

The Ptac promoter is a strong promoter in Agrobacterium strains. It can control the expression of the virE2 coding sequence. Studies have shown that virE2 expression can mediate T-DNA transfer, it requires IPTG induction and has a certain leakage^[1]. Another study tested good controllability by designing a synthetic Ptac/cuo promoter by replacing the lac operator sequence in the Ptac promoter with the cumate operator sequence^[2].

Reference

[1] de Boer, H. A., Comstock, L. J., and Vasser, M. 1983. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. U.S.A. 80:21-25.

[2] Denkovskienė E, Paškevičius Š, Werner S, Gleba Y, Ražanskienė A (2015) Inducible expression of Agrobacterium virulence gene VirE2 for stringent regulation of T-DNA transfer in plant transient expression systems. Mol Plant Microbe Interact 28(11):1247–1255.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]