Part:BBa_K4628023

Pvbp2

Promoter of Agrobacterium VirD2-Binding Protein

Usage and Biology

The Pvbp2 promoter is an inducible promoter for VirD2-Binding Protein originated from Agrobacterium tumefaciens str.C58 The Pvbp2 promoter was cloned into reporter plasmids containing Green Fluorescent Protein (GFPuv, BBa_M45116) and transformed into Agrobacterium tumefaciens GV3101 for further testing.

Acetosyringone (AS) is the most commonly used inducer for transgene induction in Agrobacterium tumefaciens, and an important function of VBP proteins is to recruit T-complexes to type IV secretion systems^[1]. So we tried to induce the expression of this promoter using acetosyringone. According to relevant literature, the concentration of the inducer was about 150μM^[2].

An examination of chromaticity values, obtained at consistent exposure times using a multi-function imager, reveals significant disparities. The basal fluorescence of the culture was subtracted in this analysis.

^FIG. Photographs of colonies under a multi-function imager (control on the right and Agrobacterium with GFP protein on the left).

^FIG. The relative fluorescence intensity of Pvbp2-GFPuv was significantly different from the control group (p<0.05)

Reference

[1] Guo ML, Hou QM, Hew CL, et al. Agrobacterium VirD2-binding protein is involved in tumorigenesis and redundantly encoded in conjugative transfer gene clusters[J]. Mol Plant Microbe Interact, 2007, 20(10):1201-1212.

[2] XU Nan, XU Yu-juan, SUN Pan, ZONG Ren-jie, GUO Min-liang. Exploration of the Transcriptional Regulation of Agrobacterium tumefaciens vbp2 Promoter[J]. Biotechnology Bulletin, 2021, 37(12): 41-49 doi:10.13560/j.cnki.biotech.bull.1985.2021-0151

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 316
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]