Part:BBa_K4628021

TMV RNA dependent RNA polymerase

Usage and Biology

The genome of tobacco mosaic virus (TMV) consists of a single-stranded RNA molecule of about 6,400 nucleotides in length with positive polarity, which encodes at least four polypeptides: 126- and 183-kDa proteins required for transcription and replication, a 30-kDa (30K) protein for cell-to-cell virus movement in infected plants, and an 18-kDa protein for virus coat formation. The sequence of the 126K protein is encoded by the 5′-proximal region of the viral genome and includes the methyltransferase and RNA helicase motifs, while the 183K protein is a read-through protein of the 126K open reading frame (ORF) and contains, in addition to the above two motifs, the RNA-dependent RNA polymerase motif. The RNA polymerase is involved in both transcription and replication. From sequence analysis, it is believed that the viral RNA polymerase contains the 183K protein as a catalytic subunit, but the precise molecular compositions of transcriptase and replicase have not yet been determined.

This Part is derived from the sequences encoding 126K and 183K proteins in the TMV genome, which has RNA-dependent RNA polymerase activity and can help the target RNA sequence replication and transcription.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal NheI site found at 1910
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal BglII site found at 2609
  Illegal BglII site found at 3344
  Illegal BglII site found at 4172
  Illegal BglII site found at 4267
  Illegal BamHI site found at 3320
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 258
  Illegal EcoRI site found at 2663
  Illegal EcoRI site found at 4242
  Illegal XbaI site found at 987
  Illegal XbaI site found at 3347
  Illegal SpeI site found at 1206
  Illegal SpeI site found at 3209
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4726